Transfection

FECT™ – siRNA transfection reagent

iFECT™ – has been used successfully to transfect siRNA into HEK293, HuH-7 (human hepatoma), B16 melanoma, CHO-K1, COS-1, COS-7, NIH3T3, SF-9, S2, primary human keratinocytes, primary rat aortic smooth muscle (WKY3M), and other cell lines. No significant toxicity was observed at the concentrations used for optimal activity on the cell lines tested. iFECT™ has been shown to be most optimal for the cell lines tested at a working concentration of 2-5 µg per µg of siRNA in 35mm dishes.

For our detailed siRNA protocol [click here]
Cationic Lipid Transfection Reagents

If you published an article using our lipid, please send us a copy of your reprint and we will send you a vial of one of our lipids (your choice) free of charge (shipping charge not included.)
Maxfect™ – DNA & oligo transfection reagent that works in serum containing media

Maxfect™ – has been used successfully to transfect short oligos and plamid DNA into CHO-K1, COS, LNCaP, HeLa, NIH3T3, SF-9, S2 (Schneider – Drosophila), 293, T-24 (human bladder carcinoma), ECV304, HCT-8 (colon carcinoma), primary human keratinocytes, primary aortic smooth muscle, primary chondrocytes, primary rabbit myoblasts, human bone marrow endothelial cells (HBMEC), HUVEC, PANC-1, THP-1, rat embryo fibroblasts, IIC9, and other cell lines. No significant toxicity was observed at the concentrations used for optimal activity on the cell lines tested. Maxfect™ has been shown to be most optimal for the cell lines tested at a working concentration of 2-6 µg per µg of DNA in 35mm dishes.
Store at 4°C, Do Not Freeze.
Genfect™ – DNA transfection reagent which works in primary cell culture

Genfect™ – has been used successfully to transfect DNA into COS, HepG2, PC12, C2C12 (mouse myoblast), Jurkat, NIH3T3, primary rat and hamster hepatocytes, primary rat cardiomyocytes, primary male rat germ cells, primary chick retinal neurons, primary astrocytes, primary rat fibroblasts, primary human keratinocytes, primary PBMC, primary cortical neurons, IM392, neuroblastoma, and tracheobroncial cells. No significant toxicity was observed at the concentrations used for optimal activity on the cell lines tested. Genfect™ has been shown to be most optimal for the cell lines tested at a working concentration of 1-4 µg per µg of DNA in 35mm dishes.
October 6, 2009 click here to see citations click here to see citations Cationic Lipid Transfection Reagents Protocol

Our detailed protocol for use with Maxfect™ and Genfect™ cationic lipid transfection reagents.
Cationic Lipid Transfection Reagents Protocol

Our detailed protocol for use with Maxfect™ and Genfect™ cationic lipid transfection reagents.
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2009

Catalog No.
Size
Price
TR-1010-1 20% OFF until 8/31/05 1mL
TR-1010-02 0.2mL
Euro269.00
Euro50.00
Quantity
Catalog No.
Size
Price
TR-1005-1 20% OFF until 8/31/05 1mL
TR-1005-02 0.2mL
Euro219.00
Euro50.00