The use of Elisa kits for the detection of proteinsis a
specific and fast method.
Enzyme-conjugated antibodies are the
basic components enabling detection and
the identification of these proteins.
Enzymes
usually attached to antibodies are the
alkaline phosphatase and peroxidase.
The realization of an Elisa test is done in a
polystyrene plate generally containing 96 wells.
WELL RECOVERY BY THE RECOVERY ANTIBODY
Each ELISA test includes:
16 wells used to double check the quality of
buffers and antibodies in a standard curb.
The first well serves as a blank to establish the value of reference for reading densities optics.
How to perform an Elisa