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Longstanding favorites of molecular biologists the world over, UreaGels 6 and 8 each consists of UreaGel Monomer Solution and UreaGel Complete Buffer Solution (formerly SequaGel 6 and SequaGel 8). The UreaGel Monomer Solution contains urea, as well as acrylamide and bis-acrylamide in the standard 19:1 ratio. UreaGel Complete Buffer Solution contains TBE and TEMED. Upon combining these two solutions, the researcher adds ammonium persulfate to form a crystal clear electrophoresis matrix with corresponding percent acrylamide (6 or 8) containing 1X TBE (89 mM Tris Base, 89 mM Boric Acid, 2 mM EDTA, pH 8.3) and 6M Urea.
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