Caspase Poly, 3 Detection

APO LOGIX Sulforhodamine Caspase Detection Kit ™
Apoptosis detection and in situ labeling of active caspase in live cells.
Key Benefits:
  • Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition.
  • Cell permeablity permits direct visualization of cytosolic apoptotic events.
  • Apoptotic cell population does not diminish over time.
  • Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure.
  • Works in diverse cell lines: human, rodent, Drosophila.
  • Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells.
  • Permits high through-put screening. Protocol can be adapted for ex vivo as well as in situ experiments.
  • Applications – Works with fluorescence microscope, 96-well fluorescence plate readers
  • Yields both quantitative and qualitative results. Gives strong signal with little background noise.
Assay Principle
APO LOGIX SR kits contain a generic sulforhodamine labeled caspase inhibitor (sulforhodamine-peptide-fluoromethyl ketone). This reagent is cell permeable and is used on whole cells to detect apoptosis. Apoptotic cells are detected by a fluorescence plate reader or fluorescence microscope using an excitation source at 550nm and measuring emission at 595nm. The assay takes about 1 hr to complete
Kit contents
  • Lyophilized SR-VAD-FMK
  • 10X Wash Buffer
  • 10X fixative
The following kits are available:
Catalog No.
(No. of Tests)
Price (Eur)
SR-VAD-FMK Poly caspase detection kit SR100-1 25 €195
SR100-2 100 €525
SR-DEVD-FMK Caspase 3 detection kit SR200-1 25 €195
SR200-2 100 $525
Jurkat cells stimulated with staurosporine for 2 hours and then labeled with SR-VAD-FMK.

Left side: 30X phase contrastRight side: 30X fluorescence microscope. Excitation: 550nm emission > 580nm.

Jurkat cells stimulated with staurosporine for 2 hours. Cells were then stained with SR-VAD-FMK for 1 hour and read in a 96 well fluorescence plate reader.