February 4, 2014

AquaPor ES GTAC

Ultra High Strength/Low EEO Agarose
Blotting of MegaBase DNA

The very low EEO of AquaPor ES will significantly reduce electrophoresis times for Pulsed Field Gel(PFG) applications, and also increase band sharpness. The extremely high gel strength makes it possible to cast gels as low as 0.3% which remain intact through staining, destaining, photographic documentation, and blotting. These low percentage gels may be used for the electrophoretic separation of DNA fragments as large as 50 kb, and macromolecular protein complexes from 500 kDa to 10 MDa size.

EEO (-m_r)	Gel Strength (g/cm²)	Gel Temp (°C)
< 0.05	> 3200 @ 1.5%	< 40 @ 1.0%
Melt Temp (°C)	Sulfate (%)	DNase, RNase, Protease
< 89 @ 1.5%	< 0.20	None

Catalog Number: EC-203

APPLICATIONS OF AQUAPOR ES GTAC

The Agarose Matrix

Agarose Gel Electrophoresis of DNA and RNA

Preparation of Agarose Gels

PFGE and FIGE

Related Products
TAE Buffer (50X) Manufactured with ultra-pure reagents, 18 megOhm water, and 0.2 micron filtration.
AquaPor LE GTAC High quality, general purpose agarose. Low boil-over. Low EEO. DNase and RNase free.
AquaPor LM GTAC Premium low melting agarose. Certified for in-gel ligation and PCR. DNase and RNase free.
AquaPor 3:1 GTAC Specialty grade agarose whose low viscosity enables pouring high percentage gels easily.
AquaPor HR GTAC National Diagnostics highest resolving agarose. Resolves down to 2% size difference.
Nuclistain Ethidium bromide substitute enabling the UV free visualization of DNA and RNA.