SequaGel MD permits minor mutational differences in DNA sequences to be detected as a high resolution relative mobility (Rf) shift. SequaGel MD is a proprietary formulation, supplied as a 2X stock, designed to resolve sequence related differences by SSCP (Single Strand Conformational Polymorphism) and Heteroduplex Analysis.
DNA mutations, or sequence modifications, are readily associated with specific disease states. Since a variation of a single nucleotide in a sequence may indicate a significant genetic anomaly, an extremely sensitive method to analyze these mutations is necessary.
Two principle methods of conformational analysis have been developed, SSCP Analysis and Heteroduplex Analysis. SequaGel MD is available as a monomer solution packaged with Triple Dye Loading Buffer in the SequaGel MD Heteroduplex Kit or with SSCP Stop Solution in the SequaGel MD SSCP Kit
SequaGel MD is designed to run SSCP samples in the 100-300 nucleotide range at a 0.5X concentration. Small fragments may be better resolved by using higher concentration (0.75X) gels. Larger fragments may be better resolved by running lower concentration (0.4X) gels. SequaGel MD is designed to run heteroduplex analysis on DNA fragments up to 900 bases at a 1X concentration.